Abstract
Introduction: Breast cancer is the most common cancer diagnosis and the leading cause of death for women worldwide. A multitude of women favor alternative therapies to address their ailments, mitigate pharmaceutical adverse effects, or postpone illness progression. Objective: This study investigates the application of economical, environmentally sustainable ethanol-extracted Fenugreek and green tea in treating breast cancer. Methods: The impact of these plant extracts on MCF-7 cells was evaluated utilizing MTT, qRT-PCR, and ELISA methodologies. The findings indicated that Fenugreek extract inflicted cellular damage. Similar to green tea, Fenugreek elevated LDH levels in cells. Fenugreek extracts induced apoptosis in MCF-7 cells more than green tea extracts. Results: Fenugreek extracts promote dendritic cell maturation by modulating the JNK, p38 MAPK, and NF-κB pathways, resulting in elevated IL-1β, IL-6, IL-8, and TNF-α levels. One of the initial RAS/RAF/MEK/ERK-associated breast tumors is associated with Raf-1 expression. In MCF-7, P38 and Raf1 exhibited down-regulation, whereas TNFα, IL1β, IL6, and IL8 shown up-regulation. Conclusion: There are no significant effects from green tea extract. Ultimately, fenugreek extract decreased apoptosis and pro-inflammatory cytokines.